MedWorm: Doxycycline

  DOXYCYCLINE HYCLATEtablet, Film Coated [IVAX Pharmaceuticals, Inc.]

Updated Date: Mar 16, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Adverse effects of drugs on the esophagus

Given the function of the esophagus to transport orally ingested solids and liquids into the stomach there are several medications with adverse effect on esophageal structures and function. Various pharmacologic agents can induce esophageal injury, promote gastroesophageal reflux by decreasing lower esophageal sphincter tone or affect esophageal perception and motility. The risks of bisphosphonates, doxycycline, ferrous sulfate, ascorbic acid, aspirin/NSAIDs and chemotherapeutic agents to induce esophageal lesions have been documented in case reports and short series. In addition to direct mucosal injury, many commonly used medications including nitroglycerins, anticholinergics, β-adrenergic agonists, aminophyllines, and benzodiazepines promote/facilitate gastroesophageal reflux by reduci...

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  Systemic isotretinoin in the treatment of rosacea – doxycycline- and placebo-controlled, randomized clinical study

Conclusions: Isotretinoin 0.3 mg/kg is an effective and well-tolerated therapy option for the treatment of rosacea subtype II and III and can therefore be used successfully as an alternative to therapy with oral antibiotics. (Source: JDDG)

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  DOXYCYCLINE HYCLATEcapsule [Contract Pharmacy Services-PA]

Updated Date: Mar 11, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Mycoplasma genitalium symptoms, concordance and treatment in high-risk sexual dyads

The objective of this study was to determine the prevalence and concordance of Mycoplasma genitalium (MG) among Mexican American and African American women and their male sexual partners. Secondary objectives were to determine symptoms of MG infection and persistence of MG after antibiotic therapy. Heterosexual couples were tested for MG and interviewed separately regarding symptoms and behavioural/epidemiologic variables at baseline, six and 12 months. The overall prevalence of MG among women and men was 9.5% and 10.6%, respectively. Subjects were five times more likely to be infected with MG if their sexual partner was MG positive. Among men and women, MG prevalence and mean bacterial loads were similar after receiving single-dose azithromycin, doxycycline or no antibiotics. MG was assoc...

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  Skin Toxicity Evaluation Protocol With Panitumumab (STEPP), a Phase II, Open-Label, Randomized Trial Evaluating the Impact of a Pre-Emptive Skin Treatment Regimen on Skin Toxicities and Quality of Life in Patients With Metastatic Colorectal Cancer [Gastrointestinal Cancer]

Conclusion The pre-emptive skin treatment regimen was well tolerated. The incidence of specific ≥ grade 2 skin toxicities during the 6-week skin treatment period was reduced by more than 50% in the pre-emptive group compared with the reactive group. Patients in the pre-emptive group reported less QOL impairment than patients in the reactive group. (Source: Journal of Clinical Oncology)

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  Scrub typhus mimicking enteric fever; a report of three patients

We report three patients who presented with fever and late onset diarrhoea mimicking enteric fever. All three patients were diagnosed with an Orientia tsutsugamushi infection and responded dramatically to doxycycline treatment. Clinicians practicing in rickettsial disease endemic areas should be made aware of similar clinical presentations in order to prevent morbidity and mortality associated with rickettsioses. (Source: Transactions of the Royal Society of Tropical Medicine and Hygiene)

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  Doxycycline-chloroquine vs. doxycycline-placebo for malaria prophylaxis in nonimmune soldiers: a double-blind randomized field trial in sub-Saharan Africa

Summary: Failures of malaria chemoprophylaxis have been related to a lack of compliance with doxycycline due to its short elimination half-life. Adding a molecule with a long half-life to doxycycline could be useful to take over from this drug in case of occasional missed doses. A double-blind, placebo-controlled randomized field trial was designed to compare the tolerability of a doxycycline-chloroquine combination vs. doxycycline as malaria prophylaxis among French soldiers deployed in Africa. Data from 936 volunteers were analyzed. In both groups, the proportion of volunteers who reported at least one adverse effect was about 57%. Tolerability was similar in the groups except for a higher proportion of nausea or vomiting in the doxycycline-chloroquine group. The reported compliance rate...

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  Severe Acne Responds to Combo Therapy (CME/CE)

Patients with severe acne had significantly greater improvement when topical adapalene/benzoyl peroxide (Epiduo) was added to oral doxycycline, according to results of a randomized clinical trial. (Source: MedPage Today Dermatology)

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  Tetracycline antibiotics in hospital and municipal wastewaters: a pilot study in Portugal.

This study investigated the occurrence of tetracyclines (TCs), namely minocycline (MIN), TC, and its epimer epitetracycline (ETC), and doxycycline (DC), in four hospital wastewater effluents and its fate in municipal wastewater treatment plants (WWTPs), in Coimbra, Portugal. Analytical determination was carried out by solid-phase extraction followed by liquid chromatography with fluorescence detection. A gradient system with a mobile phase containing oxalic acid 0.02 M and acetonitrile was used. After postcolumn derivatization with magnesium reagent, TCs were detected at lambda (exc) 386 nm and lambda (em) 500 nm. The proposed method allowed good sensitivity, accuracy, and precision. LOQs were 0.5 mug l(-1) for ETC and TC and 15 and 5 mug l(-1) for MIN and DC, respectively. The recovery va...

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  Severe Acne Responds to Combo Therapy (CME/CE, with audio)

Patients with severe acne had significantly greater improvement when topical adapalene/benzoyl peroxide (Epiduo) was added to oral doxycycline, according to results of a randomized clinical trial. (Source: MedPage Today Dermatology)

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  Severe Acne Responds to Combo Therapy (CME)

Patients with severe acne had significantly greater improvement when topical adapalene/benzoyl peroxide (Epiduo) was added to oral doxycycline, according to results of a randomized clinical trial. (Source: Modern Medicine)

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  Borrelia Burgdorferi "SENSU LAto" in Brazil: Occurence confirmed by immunohistochemistry and focus floating microscopy.

Authors: Talhari S, Santos MN, Talhari CC, Ferreira LC, Jr RM, Zelger B, Massone C, Ribeiro-Rodrigues R In the present study, we report the occurrence of Lyme's borreliosis in patients from the Brazilian Amazon Region. Borreliosis was investigated by immunohistochemistry and focus floating microscopy for Borrelia burgdorferi in skin biopsy samples from 22 patients with both clinical and histopathology evidences compatible with Erythema Migrans. Spirochetes were detected by specific immunohistochemistry and focus floating microscopy for Borrelia burgdorferi in samples from five patients. Clinical cure of the cutaneous lesions was observed in all the patients after treatment with doxycycline regimen as proposed by the Center Disease Control guidelines. A limitation of our study was the f...

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  DOXYCYCLINE HYCLATEcapsule DOXYCYCLINE HYCLATEtablet [Watson Laboratories, Inc.]

Updated Date: Mar 2, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  DOXYCYCLINE HYCLATEtablet, Film Coated [Sandoz Inc]

Updated Date: Mar 1, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli

Conclusion: This study probably suggests possibility of concurrent use of these antibiotics and plant extracts in treating infections caused by E.coli or at least the concomitant administration may not impair the antimicrobial activity of these antibiotics. (Source: BMC Complementary and Alternative Medicine)

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  Akt and c-Myc Differentially Activate Cellular Metabolic Programs and Prime Cells to Bioenergetic Inhibition [Molecular Bases Of Disease]

The high glucose consumption of tumor cells even in an oxygen-rich environment, referred to as the Warburg effect, has been noted as a nearly universal biochemical characteristic of cancer cells. Targeting the glycolysis pathway has been explored as an anti-cancer therapeutic strategy to eradicate cancer based on this fundamental biochemical property of cancer cells. Oncoproteins such as Akt and c-Myc regulate cell metabolism. Accumulating studies have uncovered various molecular mechanisms by which oncoproteins affect cellular metabolism, raising a concern as to whether targeting glycolysis will be equally effective in treating cancers arising from different oncogenic activities. Here, we established a dual-regulatable FL5.12 pre-B cell line in which myristoylated Akt is expressed under t...

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  Constitutive activation of BMP signalling abrogates experimental metastasis of OVCA429 cells via reduced cell adhesion

Conclusions: We propose that the key steps of ovarian cancer metastasis, specifically cell cohesion of multicellular aggregates in ascites and cell adhesion for reattachment to secondary sites, may be inhibited by overactive BMP signalling, thereby decreasing the ultimate malignant potential of ovarian cancer in this model system. (Source: Journal of Ovarian Research)

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  Low-quality evidence that atovaquone-proguanil and doxycycline are better tolerated prophylactic antimalarial regimens in travellers than mefloquine

(Source: Evidence-Based Nursing)

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  Galderma Announces Positive Results Of Study Evaluating A Regimen Of Epiduo Gel And Doxycycline 100mg For Patients With Significant Acne

–Results Could Help Redefine Acne Treatment Regimens and Validate Established Consensus Guidelines– Fort Worth, TX – February X, 2009 – Results from the ACCESS study found that the addition of Epiduo® (adapalene... (Source: Drugs.com - Clinical Trials)

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  Chlamydophila pneumoniae

is estimated to cause about 10% of community-acquired pneumonia (CAP) cases and 5% of bronchitis cases, although most patients with C pneumoniae infection are asymptomatic, and the course of respiratory illness is relatively mild. The incubation period of C pneumoniae infection is around 21 days, and such symptoms as cough and malaise show a gradual onset, yet may persist for several weeks or months despite appropriate antibiotic therapy. Diagnosis by nasopharyngeal specimen culture, serum antibody titers, or molecular techniques is usually delayed with respect to the onset of symptoms, antibiotic treatment, or disease resolution and there is no accurate, standardized, commercial US Food and Drug Administration–cleared diagnostic method available. Erythromycin, tetracycline, and doxycyc...

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  Psittacosis

is a systemic zoonotic infection with protean clinical features. The major risk factor is exposure to birds; bird owners, veterinarians, those involved with breeding and selling birds, and commercial poultry processors are most at risk. Patients typically present with 1 week of fevers, headache, myalgias, and a nonproductive cough. Although pneumonia is the most common manifestation, all organ systems can be involved. Serology remains the mainstay of diagnosis; however, polymerase chain reaction techniques offer a rapid and specific alternative. Doxycycline is the treatment of choice. (Source: Infectious Diseases Clinics of North America)

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  An inducible Tet-Off-H2B-GFP lentiviral reporter vector for detection and in vivo isolation of label-retaining cells.

Authors: Falkowska-Hansen B, Kollar J, Grüner BM, Schanz M, Boukamp P, Siveke J, Rethwilm A, Kirschner M Many regenerative cells are label-retaining cells (LRCs) due to their ability to keep a DNA label over a prolonged time. Until recently, isolation of vital LRCs was hampered due to the necessary use of fixation methods. To circumvent this, we generated a lentiviral-(HIV-1) based vector expressing a Tet-Off controlled histone 2B-GFP (Tet-Off-H2B-GFP) reporter gene for the detection and isolation of viable LRCs. In initial experiments, the vector was successfully used to infect 2-and 3-dimensional tissue culture models. Infected cultures from skin and pancreatic cells showed a very tight regulation of H2B-GFP, were sensitive to minimal amounts of doxycycline (Dox) and had a stabl...

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  DOXYCYCLINE (Doxycycline Hyclate) Tablet, Coated [Rebel Distributors Corp.]

Updated Date: Feb 17, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Generation and characterization of a Tet-On (rtTA-M2) transgenic rat

Conclusions: This conditional expression rat model may have application for transgenic overexpression or knockdown studies of gene function in development, disease and gene therapy. (Source: BMC Developmental Biology - Latest articles)

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  'Prion-Like' Propagation of Mouse and Human Tau Aggregates in an Inducible Mouse Model of Tauopathy.

Conclusions:Mouse tau and exogenous human tau can coaggregate in transgenic models of tauopathy. The aggregates are in dynamic equilibrium with their subunits, so that exogenous tau disappears when its expression is switched off. Once the seeds of aggregation are generated by the foreign tau species, they propagate in a 'prion-like' fashion within the cell even after the foreign tau has disappeared. PMID: 20160454 [PubMed - as supplied by publisher] (Source: Neuro-Degenerative Diseases)

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  ORACEA (Doxycycline) Capsule, Delayed Release Pellets [Physicians Total Care, Inc.]

Updated Date: Feb 12, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  DOXYCYCLINE MONOHYDRATE (Doxycycline ) Tablet DOXYCYCLINE MONOHYDRATE (Doxycycline ) Tablet [Par Pharmaceutical Inc]

Updated Date: Feb 12, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  DOXYCYCLINE (Doxycycline Hyclate ) Capsule [STAT RX USA LLC]

Updated Date: Feb 12, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Successful Treatment of Early Implant Failure: A Case Series

Conclusions: Early detection and treatment of early progressive bone loss around dental implants are the key to saving early failing implants. The author recommends reevaluation visits 4[ndash]6 weeks postimplant placement to detect any signs of early failure so that immediate treatment can be undertaken if needed. (Source: Clinical Implant Dentistry and Related Research)

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  Doxycycline: Phototoxicity: case report

(Source: Reactions)

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  Click-Chemistry-Derived Tetracycline-Amino Acid Conjugates Exhibiting Exceptional Potency and Exclusive Recognition of the Reverse Tet Repressor.

Authors: Usai I, Krueger M, Einsiedel J, Hillen W, Gmeiner P A click-chemistry-based synthesis of biologically active doxycycline-amino acid conjugates is described. Starting from 9-aminodoxycycline derivatives and complementary functionalized amino acids, ligation was accomplished by copper(I)-catalyzed azide-alkyne [3+2] cycloaddition (CuAAC). The final products were tested in a variety of TetR and revTetR systems, and the C-terminally linked phenylalanine conjugate 12 c exhibited high selectivity for revTetR over TetR. Besides the unique property of the specific effector 12 c to effectively differentiate TetR and its reverse phenotype, the test compound proved to be almost devoid of any antibacterial activity; this will be highly beneficial for future applications to control gene ex...

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  Precise conditional immortalization of mouse cells using tetracycline-regulated SV40 large T-antigen

Cellular immortalization provides a way for expansion and subsequent molecular characterization of rare cell types. Ideally, immortalization can be achieved by the reversible expression of immortalizing proteins. Here, we describe the use of conditional immortalization based on a modified tetracycline-regulated system for the expression of SV40 large T-antigen in embryonic stem (ES) cells and mice. The modified system relies on a codon improved reverse tetracycline transactivator (irtTA) fused to the ligand-binding domain (LBD) of the androgen receptor (irtTA-ABD) or of a mutated glucocorticoid receptor (irtTA-GBD*). Induction of T-antigen is conferred only after addition of two ligands, one to activate the LBD (mibolerone for irtTA-ABD or dexamethasone for irtTA-GBD*) and one to activate ...

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  Intrinsic fluoroquinolone resistance in Orientia tsutsugamushi

Abstract: Scrub typhus is a public health concern for a population of over a billion humans, with an estimated incidence of one million cases/year in endemic areas. Although doxycycline remains the standard therapy, fluoroquinolones have been used successfully in a few patients. However, there is also clinical evidence that fluoroquinolones are ineffective in the treatment of scrub typhus. To clarify this matter, we determined the in vitro susceptibility of Orientia tsutsugamushi strain Kato to ciprofloxacin and ofloxacin and sequenced the quinolone resistance-determining region (QRDR) of the gyrA gene, the target of fluoroquinolones, of 18 fresh isolates from the Lao PDR. Orientia tsutsugamushi strain Kato was resistant to ciprofloxacin and ofloxacin in vitro (minimum inhibitory concentra...

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  Haiti

the  Major disasters in the last 10 years, 2000 – 2010: 2001 – Gujarat Earthquake, India                                20,000 Deaths 2003 – Bam Earthquake, Iran                                      30,000 Deaths 2004 – Indian Ocean Earthquake and Tsunami    230,000 Deaths 2005 – Kashmir Earthquake, Pakistan                       85,000 Deaths 2005 – Hurricane Katrina, USA                                       1,300 Deaths 2008 – Sichuan China Earthquake, Chine                 70,000 Deaths 2008 – Cyclone Nargis, Burma (Myanmar)              150,000 Deaths 2010 – Haitian Earthquake, Haiti                               170,000+ Deat...

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  Estrogen Down-regulation of the Scx Gene Is Mediated by the Opposing Strand-overlapping Gene Bop1 [Gene Regulation]

This study demonstrates a novel example of hormone-stimulated intronic non-coding RNA down-regulating the expression of an opposing strand-overlapping coding gene. (Source: Journal of Biological Chemistry)

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  Detection of matrix metallopeptidase-9-like proteins in Trypanosoma cruzi.

In this study, the cell-associated and extracellular peptidases of T. cruzi grown in modified Roitman's complex (MRC) medium were analyzed by measuring peptidase activity in gelatin-containing zymograms. Our results showed that the cell-associated peptidases as well as peptidases extracellularly released by T. cruzi displayed two distinct proteolytic classes: cysteine and metallopeptidase activities. The major cysteine peptidase, cruzipain, synthesized by T. cruzi cells was detected in cellular parasite content, as a 50 kDa reactive polypeptide, after probing with anti-cruzipain antibody. In addition, metallo-type peptidases belonging to the matrix metallopeptidase-9 (MMP-9) family were revealed, after western blotting, as a 97 kDa protein band in cellular extract and an 85 kDa polypeptide...

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  Lymphogranuloma venereum in Zurich Switzerland: Chlamydia trachomatis serovar L2 proctitis among men who have sex with men.

CONCLUSIONS: Zurich has not been spared by the recent outbreaks of LGV proctitis in MSM. Anorectal LGV infections may progress to severe destructive changes, with formation of granulomas, strictures, and perirectal abscesses. Increased awareness of this problem and establishment of reliable diagnostic tools are required. PMID: 20131124 [PubMed - as supplied by publisher] (Source: Swiss Medical Weekly)

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  Constitutively active phosphatase inhibitor-1 improves cardiac contractility in young mice but is deleterious after catecholaminergic stress and with aging

In conclusion, conditional expression of I-1c or I-1S67A enhanced steady-state phosphorylation of 2 key Ca2+-regulating sarcoplasmic reticulum enzymes. This was associated with increased contractile function in young animals but also with arrhythmias and cardiomyopathy after adrenergic stress and with aging. These data should be considered in the development of novel therapies for heart failure. (Source: Journal of Clinical Investigation)

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  DOXYCYCLINE MONOHYDRATE (Doxycycline) Capsule [Watson Laboratories, Inc.]

Updated Date: Feb 2, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Doxycycline-Mediated Inhibition of Matrix Metalloproteinases Improves Healing After Rotator Cuff Repair

Conclusion/Clinical Relevance Modulation of MMP-13 activity after rotator cuff repair may offer a novel biological pathway to augment tendon-to-bone healing. (Source: The American Journal of Sports Medicine)

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  Doxycycline-Mediated Inhibition of Matrix Metalloproteinases Improves Healing After Rotator Cuff Repair

Conclusion/Clinical Relevance Modulation of MMP-13 activity after rotator cuff repair may offer a novel biological pathway to augment tendon-to-bone healing. (Source: The American Journal of Sports Medicine)

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  Formulation and Release Behavior of Doxycycline-Alginate Hydrogel Microparticles Embedded into Pluronic F127 Thermogels as a Potential New Vehicle for Doxycycline Intradermal Sustained Delivery.

Authors: Giovagnoli S, Tsai T, Deluca PP The aim of this work was the formulation and characterization of alginate (ALG)-doxycycline (DOX) hydrogel microparticles (MPs) embedded into Pluronic F127 thermogel for DOX intradermal sustained delivery. ALG-DOX MPs were formed by adding a solution of the drug into a 1.5% polymer solution while stirring. The MPs were cross-linked by dispersion into a 1.2% CaCl(2) solution. Free MPs were characterized in terms of size, drug content, and release behavior by HPLC and UV-vis. DOX and hydrogel MPs were embedded into PF127, PF127-HPMC, and PF127-Methocel thermogels. The thermogels were characterized in terms of gelling time, morphology, and release behavior. A target release period of 4-7 days was considered optimal. The hydrogel MPs were about 20 m...

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  Vein Wall Remodeling after Deep Vein Thrombosis: Differential Effects of Low Molecular Weight Heparin and Doxycycline

Conclusion: Treatment with LMWH or DOXY did not alter the size of deep vein thrombosis, mildly altered thrombus composition, and differentially affected vein wall injury, despite similar reductions in early MMP9 activity. Whether exogenous MMP inhibition affects long-term vein wall fibrosis will require further study. (Source: Annals of Vascular Surgery)

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  [Genital lymphogranuloma venereum in an HIV-1 infected patient.]

DISCUSSION: Clinical descriptions of male genital LGV are infrequent, even during the LGV proctitis epidemic seen in Western countries in recent years. A diagnosis of LGV must be considered in the presence of sexually transmitted genital lesions, even atypical, especially among HIV-infected patients. PMID: 20171433 [PubMed - as supplied by publisher] (Source: Annales de Dermatologie et de Cenereologie)

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  On-off controllable RNA hybrid expression vector for yeast three-hybrid system.

In this study, we attempted to efficiently identify false positives by introducing a tetracycline operator (tetO) motif into the RPR1 promoter of an RNA hybrid expression vector. We successfully developed a tight tetracycline-regulatable RPR1 promoter variant containing a single tetO motif between the transcription start site and the A-box sequence of the RPR1 promoter. Expression from this tetracycline-regulatable RPR1 promoter in the presence of tetracycline-response transcription activator (tTA) was positively controlled by doxycycline (Dox), a derivative of tetracycline. This on-off control runs opposite to the general knowledge that Dox negatively regulates tTA. This positively controlled RPR1 promoter system can therefore efficiently eliminate RNA-independent false positives commonly...

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  Oropharyngeal tularemia - a differential diagnosis of tonsillopharyngitis and cervical lymphadenitis.

We report on a case of oropharyngeal tularemia in an 18-year-old girl from Bavaria (Germany) who presented with tonsillopharyngitis and cervical lymphadenitis. Strongly positive serological tests and PCR detection of F. tularensis subsp. holarctica in lymph node tissue led to the diagnosis of tularemia. After long-term treatment with doxycycline, partly in combination with ciprofloxacin, the patient recovered completely. Clinical presentation, diagnostics, treatment and recent epidemiological aspects of tularemia in Europe are discussed in this case report and review of the literature. PMID: 20213378 [PubMed - as supplied by publisher] (Source: Wiener Klinische Wochenschrift)

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  Brucella melitensis infection in total knee arthroplasty: a case report

We report a case of a 63-year-old female patient who underwent a total knee arthroplasty in which the knee later became infected with Brucella melitensis. Diagnosis was made by positive culture of a sinus tract discharge. Radiological views of the knee did not show signs of implant loosening. The patient was successfully treated with rifampicin and doxycycline without surgery. Content Type Journal ArticleCategory KneeDOI 10.1007/s00167-010-1048-xAuthors Haluk Erdogan, Baskent University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology Ankara TurkeyGokhan Cakmak, Baskent University Faculty of Medicine, Department of Orthopedics and Traumatology Ankara TurkeyAskin Erdogan, Baskent University Faculty of Medicine, Department of Internal Medicine Ankara Tu...

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  Effects of MMP-9 inhibition by doxycycline on proteome of lungs in high tidal volume mechanical ventilation-induced acute lung injury

Conclusion: Administration of doxycycline might be a significant supportive therapeutic strategy in prevention of VILI. (Source: Proteome Science)

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  Members of the RSC Chromatin-remodeling Complex Are Required for Maintaining Proper Nuclear Envelope Structure and Pore Complex Localization.

Authors: Titus LC, Dawson TR, Rexer DJ, Ryan KJ, Wente SR Monitoring Editor: Yixian Zheng The assembly, distribution and functional integrity of nuclear pore complexes (NPC) in the nuclear envelope (NE) are key determinants in the nuclear periphery architecture. However, the mechanisms controlling proper NPC and NE structure are not fully defined. We used two different genetic screening approaches to identify S. cerevisiae mutants with defects in NPC localization. The first approach examined GFP-Nic96 in 531 strains from the yeast Tet-promoters Hughes Collection with individual essential genes expressed from a doxycycline-regulated promoter (TetO7-orf). Under repressive conditions, depletion of the protein encoded by 44 TetO7-orf strains resulted in mislocalized GFP-Nic96. These includ...

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  Quantitative imaging of cartilage and bone for functional assessment of gene therapy approaches in experimental arthritis

Anti-inflammatory gene therapy can inhibit inflammation driven by TNF[alpha] in experimental models of rheumatoid arthritis. However, assessment of the therapeutic effect on cartilage and bone quality is either missing or unsatisfactory. A multimodal imaging approach, using confocal laser scanning microscopy (CLSM) and micro-computed tomography (µCT), was used for gathering 3D quantitative image data on diseased and treated murine joints. As proof of concept, the efficacy of anti-TNF-based gene therapy was assessed, comparing imaging techniques with classical investigations. SCID mice knees were injected with human synoviocytes overexpressing TNF[alpha]. Two days later, electric pulse-mediated DNA transfer was performed after injection of the pGTRTT-plasmid containing a dimeric soluble-TN...

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  Postnatally Induced Over-Expression of FGF10 Promotes Murine Hepatic Progenitor Cell Expansion Similar to the DDC Diet Model

Conclusions: Our data indicate that prenatal HPC persist after birth but undergo attrition as the liver matures. Induced Fgf10 over-expression promotes the expansion of small periportal HPC. Our IF analyses suggest that there is dual expansion of not only ALBUMIN+ hepatocyte precursors but also 3C7+PCK+ (or 6E10+PCK+) bile duct epithelial precursors. (Source: Journal of Surgical Research)

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  DARPP-32 Expression Promotes the Activation of Akt and Is Involved in the Gastric Tumorigenesis Cascade

In this study, we aimed to determine the role of DARPP-32 in the gastric tumorigenesis cascade using in vivo and in vitro approaches. Methods: Quantitative real-time RT-PCR analysis of DARPP-32 in 159 patient samples (106 with gastric adenocarcinoma and 53 with normal gastric tissue) was compared to HPRT. DARPP-32 intensity and frequency on immunohistochemistry were studied using a tumor microarray containing samples of normal stomach (118), gastritis (53), metaplasia (77), dysplasia (24), and adenocarcinoma (219). Composite expression score (CES) was calculated from intensity and frequency and correlated with histology, differentiation, and tumor type (intestinal vs. diffuse). Paired samples (i.e. normal + adenocarcinoma) were used to determine likelihood of increasing DARPP-32 CES with t...

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  The Role of Doxycycline as an Inhibitor of Intimal Hyperplasia after Balloon Angioplasty Arterial Injury

Objective: Intimal hyperplasia (IH) is the most common indicator for secondary intervention in peripheral vascular disease. The major cellular processes contributing to IH development are extracellular matrix (ECM) degradation and vascular smooth muscle cell (VSMC) migration and proliferation. Matrix metalloproteinases (MMPs) are implicated in vascular remodeling due to their ability to selectively degrade components of the ECM. Members of the tetracycline family of antibiotics are potent MMP inhibitors, doxycycline being one of the more potent inhibitors with better absorption and a longer half-life than the parent compound. We have previously shown doxycycline inhibits MMP activity and VSMC migration in vitro. Moreover, doxycycline has been found to inhibit cell proliferation in other ce...

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  Expression of COX-2 Increases Barrier Permeability in Caco-2 Tight Monolayers

Conclusion: Expression of COX-2 disrupts epithelial barrier in Caco-2 monolayers. This result suggests that PGE2 produced by the intestinal epithelium may be responsible for gut barrier breakdown during NEC. (Source: Journal of Surgical Research)

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  Comparisons of the effects of systemic administration of L-thyroxine and doxycycline on orthodontically induced root resorption in rats.

Authors: Baysal A, Uysal T, Ozdamar S, Kurt B, Kurt G, Gunhan O The aim of this study was to histologically evaluate and compare the effects of the systemic administration of l-thyroxine (TX) and doxycycline (DX) on orthodontically induced root resorption, Twenty-eight male 50- to 60-day-old Wistar rats were used. Seven rats served as the baseline control. Seven animals received TX (20 mug/kg bodyweight/day) and seven DX (1.2 mg/kg bodyweight/day), by means of a mini-osmotic pump implanted subcutaneously. Seven rats were separated as a sham, in order to evaluate the pure effect of the surgical procedure on the animals' health. Tooth movement (TM) was achieved with a continuous force of 50 g by placing Elgiloy coil springs between the right maxillary first molar and incisors for 14 days...

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  Cyclic AMP-mediated endocytosis of intestinal epithelial NHE3 requires binding to synaptotagmin 1

The apical membrane Na+-H+ exchanger (NHE)3 is regulated by cAMP-dependent phosphorylation, which inhibits its activity through membrane endocytosis. The clathrin complex adaptor protein synaptotagmin 1 (Syt 1) appears to be essential to this process, but little is known about its expression in intestinal epithelial cells or interaction with NHE3. The intestinal epithelial expression and apical location of Syt 1 were determined by Syt 1 mRNA profiling and immunolocalization. Tandem mass spectrometry was used for protein identification. Bis(sulfosuccinimidyl) suberate (BS3) cross linking suggested that NHE3 and Syt 1 were in a membrane complex following cAMP stimulation of Caco2BBE (Brush Border Expressions) cells. To investigate the regulation of NHE3 appearance in a Syt 1-containing membr...

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  Doxycycline for Mansonella perstans Infection

To the Editor: The article by Coulibaly et al. (Oct. 8 issue)1 and the corresponding editorial by Hoerauf2 point out ... (Source: New England Journal of Medicine)

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  DOXYCYCLINE Capsule DOXYCYCLINE Capsule [Par Pharmaceutical Inc.]

Updated Date: Jan 19, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  What are the Risks of Infertility after Pelvic Inflammatory Disease?

This study included patients 14-37 years but without subgroup analysis of adolescents. In another study, these researchers found that barrier methods (especially male condoms) significantly helped to prevent chronic pelvic pain, recurrent PID, and infertility. Questions for Further Discussion 1. What are the recommended treatment options for PID? 2. What are the recommendations for treating partners of patients with known PID and how are the partners contacted locally? 3. What are the legal requirements for treating minors with sexually transmitted infections? Related Cases Disease: Pelvic Inflammatory Disease | Chlamydia Infections Symptom/Presentation: Abdominal Pain | Pelvic Pain | Vaginal Discharge Specialty: Adolescent Medicine | Emergency Medicine | Infectious Diseases | Obstetrics...

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  Stability of Doxycycline and Chlorhexidine Absorbed on Root Canal Dentin

Conclusions: Compared with doxycycline, chlorhexidine is more stable on root canal dentin. (Source: Journal of Endodontics)

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  DORYX (Doxycycline Hyclate) Tablet, Delayed Release [Warner Chilcott (US), LLC]

Updated Date: Jan 14, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Antibiotic Treatment Duration and Long‐Term Outcomes of Patients with Early Lyme Disease from a Lyme Disease–Hyperendemic Area

Conclusions. Patients treated for ⩽10 days with antibiotic therapy for early Lyme disease have long‐term outcomes similar to those of patients treated with longer courses. Treatment failure after appropriately targeted short‐course therapy, if it occurs, is exceedingly rare. (Source: Clinical Infectious Diseases Latest Issue)

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  VIBRAMYCIN (Doxycycline Hyclate) Tablet [Keltman Pharmaceuticals Inc.]

Updated Date: Jan 13, 2010 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Fibrin ring granulomas in Rickettsia typhi infection

We describe a 71-year-old man hospitalized for fever and productive cough. Laboratory investigation showed anemia, thrombocytopenia, elevated transaminases, hyponatremia, and hypoalbuminemia. Computerized tomography of the abdomen, thorax, and sinuses, echocardiography, and a gallium scan did not reveal the source of the fever. The patient remained febrile despite courses of piperacillin–tazobactam/azithromycin and ceftriaxone/vancomycin. A bone marrow biopsy showed fibrin ring granulomas, and 2 rickettsial serologic panels were positive for Rickettsia typhi infection and negative for Q fever. The patient was given doxycycline, and the fever resolved within 48 h. We propose that fibrin ring granulomas also occur in murine typhus. (Source: Diagnostic Microbiology and Infectious Disease)

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  Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions

Lentiviral-mediated gene transfer in vivo or in cultured mammalian neurons can be used to address a wide variety of biological questions, to design animal models for specific neurodegenerative pathologies, or to test potential therapeutic approaches in a variety of brain disorders. Lentiviruses can infect nondividing cells, thereby allowing stable gene transfer in postmitotic cells such as mature neurons. An important contribution has been the use of inducible vectors: the same animal can thus be used repeatedly in the doxycycline-on or -off state, providing a powerful mean for assessing the function of a gene candidate in a disorder within a specific neuronal circuit. Furthermore, lentivirus vectors provide a unique tool to integrate siRNA expression constructs with the aim to locally kno...

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  Doxycycline Regulated Lentiviral Vectors

Lentiviral vectors are a powerful tool to achieve regulated expression of transgenes in vivo and in vitro. The doxycycline-inducible system is well characterized and can be used to regulate expression mediated by lentiviral vectors. Because many different doxycycline-inducible lentiviral vectors have been described, choosing the best vector system can be difficult. This chapter can be used as a guide to select the optimal system for a particular application. (Source: Springer protocols feed by Cell Biology)

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  LEFTY, a Member of the Transforming Growth Factor-{beta} Superfamily, Inhibits Uterine Stromal Cell Differentiation: A Novel Autocrine Role.

Authors: Tang M, Naidu D, Hearing P, Handwerger S, Tabibzadeh S LEFTY is expressed in normal endometrium in cells that decidualize. To understand the importance of this expression, we have studied the effect of LEFTY on decidualization in vitro and in vivo. Exposure of human uterine fibroblast (HuF) cells to recombinant LEFTY blocked the induction of the decidual differentiation-specific marker genes, IGFBP1 (IGF-binding protein 1) and PRL (prolactin) in response to medroxyprogesterone acetate, estradiol, and prostaglandin E2. The inhibitory effect was associated with decreased induction of the transcription factors ETS1 and FOXO1, both of which are essential for decidualization. Overexpression of LEFTY in decidualized HuF cells with an adenovirus that transduced LEFTY caused a marked ...

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  Severe ehrlichia infection in pediatric oncology and stem cell transplant patients

We report three cases of ehrlichiosis in pediatric oncology patients, one of whom was a stem cell transplant recipient. Early symptoms included fever, malaise, and vague gastrointestinal symptoms. Laboratory abnormalities were initially attributed to chemotherapy toxicity. Illness was severe in all three patients and one patient died even after initiation of doxycycline. These cases emphasize the need for a high index of suspicion for tickborne illness in oncology patients, and the importance of a low threshold for starting empiric treatment before confirming the diagnosis. Pediatr Blood Cancer. © 2010 Wiley-Liss, Inc. (Source: Pediatric Blood and Cancer)

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  Expression of estrogen receptor alpha with a Tet-off adenoviral system induces G0/G1 cell cycle arrest in SKBr3 breast cancer cells.

In this study, we have built on previous studies by developing a Tet-off adenoviral system to express ERalpha in the ER-negative SKBr3 breast cancer cells that over-express both EGFR and HER2. This system efficiently delivers ERalpha and the expression level of ERalpha is controlled by doxycycline in a concentration-dependent manner. The growth of SKBr3 was inhibited by ERalpha expression and further inhibited in the presence of 1 nM 17beta-estradiol. SKBr3 cells were arrested at G0/G1 cell cycle upon ERalpha expression, which corresponded to an increase of p21Cip1/Waf1, hypo-phosphorylation of pRb and decrease of E2F1. Estrogen also reduced EGFR and HER2 expression in SKBr3 cells after ERalpha was expressed. Given that estrogen-induced increase of p21Cip1/Waf1 and decrease of E2F1 was als...

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  Doxycycline inhibits proinflammatory cytokines but not acute cerebral cytogenesis after hypoxia-ischemia in neonatal rats.

Conclusion: Taken together, our results suggest the putative clinical potential of DOXY in the management of neonatal cerebral HI injury. PMID: 20040243 [PubMed - in process] (Source: J Psychiatry Neurosc...)

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  [Severe drug hypersensitivity reaction (DRESS syndrome) to doxycycline.]

CONCLUSION: This patient is the third case of DRESS to doxycycline described in the literature. The originality of this case lies in the allergological investigation using patch-tests and HLA determination. PMID: 20110067 [PubMed - as supplied by publisher] (Source: Annales de Dermatologie et de Cenereologie)

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  Native Valve Brucella Endocarditis

We report the medical and surgical management of 31 cases of native endocarditis.Thirty-one patients were admitted to our clinic with suspected Brucella Endocarditis. The diagnosis was established by either isolation of Brucella species, or the presence of antibodies. Following preoperative antibiotic therapy patients underwent valve replacement with excessive tissue debridment. Patients were followed up with Brucella titers, blood cultures, and echocardiography.On admission all patients were febrile and mostly dyspneic (NYHA Class 3 or 4). The blood tests were normal except for elevated ESR, CRP and serological tests. The aortic valve was involved in 19 patients, mitral valve in 7 patients, and both valves in 5. After serological confirmation of BE, antibiotic therapy was maintained. Twen...

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  Inhibition of PI3K by PX-866 Prevents Transforming Growth Factor-{alpha}-Induced Pulmonary Fibrosis.

Authors: Le Cras TD, Korfhagen TR, Davidson C, Schmidt S, Fenchel M, Ikegami M, Whitsett JA, Hardie WD Transforming growth factor-alpha (TGFalpha) is a ligand for the epidermal growth factor receptor (EGFR). EGFR activation is associated with fibroproliferative processes in human lung disease and animal models of pulmonary fibrosis. EGFR signaling activates several intracellular signaling pathways including phosphatidylinositol 3'-kinase (PI3K). We previously showed that induction of lung-specific TGFalpha expression in transgenic mice caused progressive pulmonary fibrosis over a 4-week period. The increase in levels of phosphorylated Akt, detected after 1 day of doxycycline-induced TGFalpha expression, was blocked by treatment with the PI3K inhibitor, PX-866. Daily administration of P...

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  Reduced Viability of Mice with Lung Epithelial-Specific Knockout of Glucocorticoid Receptor.

Authors: Manwani N, Gagnon S, Post M, Joza S, Muglia L, Cornejo S, Kaplan F, Sweezey NB Glucocorticoid (GC)-responsive epithelial-mesenchymal interactions regulate lung development. The GC receptor (GR) mediates GC signaling. Mice lacking GR in all tissues die at birth of respiratory failure. In order to determine the specific need for epithelial GR in lung development, we bred triple transgenic mice that carry SPC/rtTA, tet-O-Cre and floxed, but not wild type, GR genes. When exposed to doxycycline in utero, triple transgenic (GRepi -) mice exhibit a Cre-mediated recombination event that inactivates the floxed GR gene in airway epithelial cells. Immunofluorescence confirmed the elimination of GR in Cre-positive, airway epithelial cells of late gestation GRepi - mice. Embryonic day (E) ...

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  Peritoneal dialysis-related peritonitis caused by Brucella melitensis

We report two cases of CAPD-related peritonitis caused by Brucella melitensis. In both patients, peritonitis accompanied systemic brucellosis and was successfully treated with antibiotics (rifampicin plus doxycycline for 6 weeks) without removal of the Tenckhoff catheter. (Source: Dialysis and Transplantation)

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  In Vitro Activity of CEM-101, a New Fluoroketolide Antibiotic, against Chlamydia trachomatis and Chlamydia (Chlamydophila) pneumoniae.

Authors: Roblin PM, Kohlhoff SA, Parker C, Hammerschlag MR The in vitro activities of CEM-101, telithromycin, azithromycin, clarithromycin, and doxycycline were tested against 10 isolates each of Chlamydia trachomatis and Chlamydia (Chlamydophila) pneumoniae. The MIC at which 90% of the isolates of both C. trachomatis and C. pneumoniae were inhibited and the minimal bactericidal concentration at which 90% of the isolates were killed by CEM-101 were 0.25 mug/ml (range 0.125-0.5 mug/ml for C. trachomatis and 0.25-1.0 mug/ml for C. pneumoniae). PMID: 20038627 [PubMed - as supplied by publisher] (Source: Antimicrobial Agents and Chemotherapy)

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  Anti-invasion and anti-tumor growth effect of doxycycline treatment for human oral squamous-cell carcinoma – In vitro and in vivo studies

The objective of this study was to evaluate the effect of Dox on the expression of MMPs in vitro using the SCC-15 cell line and in vivo SCC-15 xenografted nude mice. SCC-15 cells maintained under distinct culture conditions expressed high levels of pro-MMP-2 and pro-MMP-9; however, as determined by zymography and Western blot analysis, Dox significantly reduced the production of pro-MMP-2 and pro-MMP-9 after 24h of treatment in a dose-dependent manner (2.5–40μg/ml). Dox (10μg/ml) decreased the expression of MMP-9 mRNA but did not alter the level of MMP-2 mRNA after 24h of treatment. In addition, this drug significantly inhibited the invasive and migration activities of SCC-15 cells in vitro (>75% inhibition at 10μg/ml). On the other hand, daily administration of Dox (3mg/mice) restrai...

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  Antibiotic sensitivity pattern of gram negative bacilli isolated from the lower respiratory tract of ventilated patients in the intensive care unit

Conclusion:</b> Nonfermenters are the most common etiological agents of LRTIs in ICU. There is an alarmingly high rate of resistance to cephalosporin and β-lactam-β-lactamase inhibitor group of drugs. Meropenem was found to be the most sensitive drug against all GNB. <i>Acinetobacter </i>and<i> Klebsiella </i>spp. showed good sensitivity to doxycycline. (Source: Indian Journal of Critical Care Medicine)

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  Subjective Symptoms after Treatment of Early Lyme Disease

Conclusion: No significant differences were identified between doxycycline and cefuroxime axetil in the treatment of European patients with erythema migrans. The frequency of nonspecific symptoms in patients did not exceed that of a control group at ≥6 months after enrollment. We advocate inclusion of appropriate non-Lyme disease control groups in future studies in which nonspecific subjective symptoms are assessed after antibiotic therapy. (Source: The American Journal of Medicine)

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  DOXYCYCLINE HYCLATEcapsule [Blenheim Pharmacal, Inc.]

Updated Date: Dec 23, 2009 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Cochrane review: Doxycycline for osteoarthritis of the knee or hip

Source: Cochrane Library Area: Evidence > Drug Specific Reviews Background Osteoarthritis is a chronic joint disease that involves degeneration of articular cartilage. Pre-clinical data suggest that doxycycline might act as a disease-modifying agent for the treatment of osteoarthritis, with the potential to slow cartilage degeneration.   Objectives To examine the effects of doxycycline compared with placebo or no intervention on pain and function in patients with osteoarthritis of the hip or knee.   Search strategy We searched CENTRAL (The Cochrane Library 2008, issue 3), MEDLINE, EMBASE and CINAHL up to 28 July 2008, checked conference proceedings, reference lists, and contacted authors.   Selection criteria We included studies if they were randomised or qua...

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  Cochrane review: Drugs for preventing malaria in travellers

Source: Cochrane Library Area: Evidence > Drug Class Focused Reviews Background Malaria infects 10,000 to 30,000 international travellers each year. It can be prevented through anti-mosquito measures and drug prophylaxis. However, antimalaria drugs have adverse effects which are sometimes serious.   Objectives To compare the effects of currently used antimalaria drugs when given as prophylaxis to non-immune adult and child travellers who are travelling to regions with Plasmodium falciparum resistance to chloroquine. Specifically, to assess the efficacy, safety, and tolerability of atovaquone-proguanil, doxycycline, and mefloquine compared to each other, and also when compared to chloroquine-proguanil and to primaquine.   Search strategy In August 2009 we searched the C...

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  This Month in Gastroenterology

Whipple's disease is a rare inflammatory disorder caused by infection of different organ systems with Tropheryma whipplei. In the gastrointestinal tract, jejunal infection with T whipplei causes diarrhea or steatorrhea with weight loss. Treatment with a combination of doxycycline, hydroxychloroquine, and sulfamethoxazole or sulfadiazine is usually recommended, but prospective data assessing the efficacy of these antibiotics are lacking. Moreover, retrospective case series report failure rates of up to 30% using these antibiotic regimens. (Source: Gastroenterology)

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  Role of matrix metalloproteinase-2 in the cardioprotective effect of ischaemic postconditioning

The activation of matrix metalloproteinases (MMPs) contributes to myocardial injury at the onset of reperfusion; however, their role in ischaemic postconditioning is unknown. The aim of the present study was to examine the effects of ischaemic postconditioning on MMP activity in isolated rabbit hearts. The isolated rabbit hearts were subjected to 30 min of global ischaemia followed by 180 min of reperfusion (I/R group; n= 8). In the ischaemic postconditioning group (n= 8), a postconditioning protocol was performed (2 cycles of 30 s reperfusion[ndash]ischaemia). In other experiments, we added doxycycline, an MMP inhibitor, at 25 (n= 7) or 50 [mu]mol l[minus]1 (n= 8) during the first 2 min of reperfusion. Coronary effluent and left ventricular tissue were collected during pre-ischaemic condi...

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  [Comment] A protocol for labrador retrievers?

On Christmas Day, 2000, Navy, a golden retriever aged 18 months, began a cocktail of celecoxib, tamoxifen, and doxycycline to target blood vessels supplying a tumour on her chest. Her owner Marion Haber, a veterinary student at Tufts University, Boston, MA, USA, was acquainted with the work of angiogenesis pioneer Judah Folkman at the nearby Children's Hospital, Harvard Medical School. Soon, the golden retriever became a golden guineapig. The treatment was blinded to the patient, who unknowingly polished off the regimen with her dog biscuits. 3 months later, Navy was cancer free, and the following year a healthy report was published in a US national newspaper. The excitement around the story led to the drug combination being renamed the Navy protocol, which remains a familiar term in veter...

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  Degradation of tetracycline antibiotics: Mechanisms and kinetic studies for advanced oxidation/reduction processes.

This study involves elucidating the destruction mechanisms of four tetracyclines via reactions with ()OH and solvated electrons (e(aq)(-)). The first step is to evaluate the bimolecular rate constants for the reaction of ()OH and e(aq)(-). Transient absorption spectra for the intermediates formed by the reaction of ()OH were also measured over the time period of 1-250mus to assist in selecting the appropriate wavelength for the absolute bimolecular reaction rate constants. For these four compounds, tetracycline, chlortetracycline, oxytetracycline, and doxycycline, the absolute rate constants with ()OH were (6.3+/-0.1)x10(9), (5.2+/-0.2)x10(9), (5.6+/-0.1)x10(9), and (7.6+/-0.1)x10(9) M(-1) s(-1), and for e(aq)(-) were (2.2+/-0.1)x10(10), (1.3+/-0.2)x10(10), (2.3+/-0.1)x10(10), and (2.5+/-0...

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  Inhibition of matrix metalloproteinase activity in vivo protects against vascular hyporeactivity in endotoxemia

This study demonstrates the in vivo protective effect of the MMP inhibitor doxycycline against the development of vascular hyporeactivity in endotoxemic rats. (Source: AJP: Heart and Circulatory Physiology)

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  Tetracycline Bone Fluorescence: A Valuable Marker for Osteonecrosis Characterization and Therapy

Conclusions: Given that viable but not necrotic bone displays tetracycline fluorescence, a notion that reflects the histopathology, more precise characterization of the 2 osteonecrosis types is enabled. Furthermore, even in extended cases of osteonecrosis requiring partial mandibulectomy, bone fluorescence helps to pinpoint the margins of resection and thus signifies an improvement of surgical therapy of extended osteonecrosis. (Source: Journal of Oral and Maxillofacial Surgery)

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  Inducible cardiomyocyte-specific gene disruption directed by the rat Tnnt2 promoter in the mouse

We developed a conditional and inducible gene knockout methodology that allows effective gene deletion in mouse cardiomyocytes. This transgenic mouse line was generated by coinjection of two transgenes, a "reverse" tetracycline-controlled transactivator (rtTA) directed by a rat cardiac troponin T (Tnnt2) promoter and a Cre recombinase driven by a tetracycline-responsive promoter (TetO). Here, Tnnt2-rtTA activated TetO-Cre expression takes place in cardiomyocytes following doxycycline treatment. Using two different mouse Cre reporter lines, we demonstrated that expression of Cre recombinase was specifically and robustly induced in the cardiomyocytes of embryonic or adult hearts following doxycycline induction, thus, allowing cardiomyocyte-specific gene disruption and lineage tracing. We als...

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  Post exposure prophylaxis of tick-borne relapsing fever

We examined the safety and effectiveness of this policy in preventing TBRF. We analyzed the records from January 2004 to January 2007, and identified all reported events of tick bites or TBRF cases. Data were available on 27 events in which 816 soldiers have undergone physical examination following exposure, and seven TBRF cases were recorded in this group—an attack rate of 0.86% compared with the expected rate of 5.34% from previous army data (relative risk [RR] = 0.16). Of those screened, 128 (15.7%) had tick-bite and were intended for prophylaxis, of which four TBRF cases occurred—3.13% attack rate compared with an expected rate of 38.4% in these bitten individuals without prophylaxis (RR = 0.08, number needed to treat = 3). In all cases in which screening and prophyla...

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  Single-gene transgenic mouse strains for reprogramming adult somatic cells

We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds. (Source: Nature Methods)

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  High-throughput analysis of tetracycline antibiotics and their epimers in liquid hog manure using Ultra Performance Liquid Chromatography with UV detection.

Authors: Tylová T, Olšovská J, Novák P, Flieger M Antibiotics contained in animal manure can contaminate soil, groundwater and eventually surface and drinking water. To reduce the usage of antibiotics in livestock industry the EU banned their application as growth promoters in 2006. Even though the antibiotics are still used for this purpose and therefore it is necessary to control their applications. An Ultra Performance Liquid Chromatography method (UPLC) with UV detection for determination of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), and doxycycline (DOX) including their epimers in the liquid hog manure was developed. The antibiotics were extracted with ethyl acetate and separated on UPLC BEH Shield RP18 column. The validated method was ...

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  Increased incidence of postoperative infections during prophylaxis with cephalothin compared to doxycycline in intestinal surgery

Conclusions: Doxycycline combined with metronidazole, is an attractive candidate for antibiotic prophylaxis in medium and major size intestinal surgery. (Source: BMC Surgery)

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  IL-17 mediates articular hypernociception in antigen-induced arthritis in mice

In this study, we evaluated the role of IL-17 in the genesis of articular nociception in a model of antigen (mBSA)-induced arthritis. We found that mBSA challenge in the femur–tibial joint of immunized mice induced a dose- and time-dependent mechanical hypernociception. The local IL-17 concentration within the mBSA-injected joints increased significantly over time. Moreover, co-treatment of mBSA challenged mice with an antibody against IL-17 inhibited hypernociception and neutrophil recruitment. In agreement, intraarticular injection of IL-17 induced hypernociception and neutrophil migration, which were reduced by the pre-treatment with fucoidin, a leukocyte adhesion inhibitor. The hypernociceptive effect of IL-17 was also reduced in TNFR1−/− mice and by pre-treatment with infliximab...

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  DOXYCYCLINE HYCLATEcapsule [West-Ward Pharmaceutical Corp]

Updated Date: Dec 4, 2009 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  Imbalance between matrix metalloproteinases and tissue inhibitor of metalloproteinases in hypertensive vascular remodeling.

Authors: Castro MM, Rizzi E, Prado CM, Rossi MA, Tanus-Santos JE, Gerlach RF Structural vascular changes in two-kidney, one-clip (2K-1C) hypertension may result from increased matrix metalloproteinase (MMP)-2 activity. MMP-2 activation is regulated by other MMPs, including transmembrane-MMPs, and by tissue inhibitors of MMPs (TIMPs). We have investigated the localization of MMP-2, -9, -14, and TIMPs 1-4 in hypertensive aortas and measured their levels by zymography/Western blotting and immunohistochemistry. Gelatinolytic activity was assayed in tissues by in situ zymography. Sham-operated and 2K-1C hypertensive rats were treated with doxycycline (or vehicle) for 8weeks, and the systolic blood pressure was monitored weekly. Doxycycline attenuated 2K-1C hypertension (165+/-11.7mmHg versu...

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  TC-PTP is required for the maintenance of MYC-driven B-cell lymphomas

We sought to determine the contributions of protein tyrosine phosphatases (PTPs) to the pathogenesis of B-cell lymphomas. We found that T-cell PTP (TC-PTP) was overexpressed in transformed B cells. We hypothesized that TC-PTP may be a tumor-promoting gene that is regulated by MYC overexpression in B cells. Knockdown of TC-PTP in murine tumors resulted in decreased cell viability in vitro because of an arrest in the G1 phase of the cell cycle. Furthermore, cells with reduced TC-PTP expression were unable to either engraft or expand in vivo. Taken together, these data indicate that TC-PTP is required for B-cell tumor maintenance. Our data also suggested a correlation between TC-PTP expression and MYC overexpression. To investigate this further, we used malignant murine B cells that contain a...

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  Abstract A53: Dose-dependent levels of p53 mediate different cell fate decisions in human mammary epithelial cells

Conclusions: Our results indicate that the level of p53 expression plays an important role in cell fate decisions between arrest and apoptosis. Furthermore, our microarray data suggest that high physiological levels of p53 regulate a specific subset of target genes associated with p53-dependent cell death.Citation Information: Cancer Res 2009;69(23 Suppl):A53. (Source: Cancer Research)

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  A sugar coated solution - covering letter

The clinical presentation, combined with uptake in the bone marrow on 18F-FDG PET, prompted a bone marrow biopsy, which showed ‘ring' or ‘doughnut' granulomata, typical of Q fever. The patient commenced doxycycline therapy, with rapid defervescence and normalisation of inflammatory markers. In retrospect, he recalled close contact with a herd of goats during his trip to China. This is the first reported case of detection by 18F-FDG PET of bone marrow infiltration in Q fever. The importance of bone marrow biopsy in the investigation of fever of unknown origin is emphasised. (Source: Journal of Infection)

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  Modulation of metalloproteinase activity in tuberculosis by Interleukin-17 and anti-tuberculous drugs

Tuberculosis kills 2 million people every year. Matrix Metalloproteinases (MMPs) are implicated in tuberculous tissue destruction. We investigated the effect of the Th17 cytokine, Interleukin-17, which contributes to anti-mycobacterial immunity, on MMPs in an in vitro model. Primary airway epithelial cells were stimulated with Interleukin-17, with or without CoMTB (conditioned medium from TB infected monocytes). CoMTB and Interleukin-17 synergistically up-regulated MMP-1 (interstitial collagenase) and MMP-3 (stromelysin) secretion. In contrast, Interleukin-17 down-regulated MMP-9 (gelatinase) secretion. On western analysis, IL-17 drove AkT (Protein Kinase B) phosphorylation. Interleukin-17 and CoMTB driven MMP-3 secretion was abolished by chemical inhibition of the Pi3Kinase pathway and fu...

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  Low-Dose Doxycycline Sufficient in Rosacea

The jury is still out on whether rosacea has a microbial etiology, but one thing is certain: Low-dose doxycycline is as effective as higher doses, with significantly fewer side effects, Dr. Guy Webster reported at a dermatology seminar sponsored by Skin Disease Education Foundation. (Source: Internal Medicine News)

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  Current treatment of autoimmune blistering diseases.

Authors: Kasperkiewicz M, Schmidt E Autoimmune bullous diseases are a heterogeneous group of disorders that can be subdivided according to the level of split formation in the intraepidermal blistering pemphigus diseases and subepidermal bullous disorders, latter including pemphigoid diseases, epidermolysis bullosa acquisita, and dermatitis herpetiformis. In the majority of autoimmune bullous disorders, disease activity can be sufficiently controlled by systemic corticosteroids in combination with further immunosuppressants/immunomodulants such as dapsone, doxycycline, methotrexate, azathioprine, or mycophenolate mofetil. In contrast, in pemphigus, mucous membrane pemphigoid, and epidermolysis bullosa acquisita, treatment is challenging and only in a minority of patients, conventional i...

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  Inducible Nephrin Transgene Expression in Podocytes Rescues Nephrin-Deficient Mice from Perinatal Death.

Authors: Juhila J, Lassila M, Roozendaal R, Lehtonen E, Messing M, Langer B, Kerjaschki D, Verbeek JS, Holthofer H Mutations leading to nephrin loss result in massive proteinuria both in humans and mice. Early perinatal lethality of conventional nephrin knockout mice makes it impossible to determine the role of nephrin protein in the adult kidney and in extra-renal tissues. Herein, we studied whether podocyte-specific, doxycycline-inducible, rat nephrin expression can rescue nephrin-deficient mice from perinatal lethality. Fourteen littermates out of 72 lacked endogenous nephrin and expressed transgenic rat nephrin. Six of these rescued mice survived until 6 weeks of age, whereas the nephrin-deficient pups died before the age of 5 days. The rescued mice were smaller, developed proteinu...

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  Glyceraldehyde-3-phosphate Dehydrogenase Aggregate Formation Participates in Oxidative Stress-induced Cell Death [Mechanisms Of Signal Transduction]

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)2 is a classic glycolytic enzyme that also mediates cell death by its nuclear translocation under oxidative stress. Meanwhile, we previously presented that oxidative stress induced disulfide-bonded GAPDH aggregation in vitro. Here, we propose that GAPDH aggregate formation might participate in oxidative stress-induced cell death both in vitro and in vivo. We show that human GAPDH amyloid-like aggregate formation depends on the active site cysteine-152 (Cys-152) in vitro. In SH-SY5Y neuroblastoma, treatment with dopamine decreases the cell viability concentration-dependently (IC50 = 202 µm). Low concentrations of dopamine (50–100 µm) mainly cause nuclear translocation of GAPDH, whereas the levels of GAPDH aggregates correlate...

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  Susceptibility of Plasmodium falciparum Isolates to Doxycycline Is Associated with pftetQ Sequence Polymorphisms and pftetQ and pfmdt Copy Numbers

The Journal of Infectious Diseases, Volume 0, Issue 0, Page 000, Latest Articles. Background. Doxycycline is used in combination with quinine for malaria treatment or alone for malaria chemoprophylaxis. However, the occurrence of malaria after doxycycline chemoprophylaxis has been reported. Identification of genetic determinants that contribute to the susceptibility of Plasmodium falciparum to doxycycline will be important for the detection and surveillance of doxycycline resistance. Methods. Sequence analysis of 11 genes (pftufA, pfEF‐TS, pfmdt, pftetQ, pfrps3, pfrps7, pfrps8, pfrps9, pfrps11, pfrps14, and pfrps17) and evaluation of pfmdt and pftetQ copy numbers by quantitative real‐time polymerase chain reaction were conducted in 90 African P. falciparum isolates that wer...

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  Prevention of Cardiac Dysfunction in Acute Coxsackievirus B3 Cardiomyopathy by Inducible Expression of a Soluble Coxsackievirus-Adenovirus Receptor.

Conclusion-AdG12-mediated sCAR-Fc delivery prevents cardiac dysfunction in CVB3 myocarditis under prophylactic and therapeutic conditions. PMID: 19933937 [PubMed - as supplied by publisher] (Source: Circulation)

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  Cyclic AMP-mediated endocytosis of intestinal epithelial NHE3 requires binding to synaptotagmin 1.

Authors: Musch MW, Arvans DL, Wang Y, Nakagawa Y, Solomaha E, Chang EB The apical membrane Na(+)-H(+) exchanger NHE3 is regulated by cAMP-dependent phosphorylation, which inhibits its activity through membrane endocytosis. The clathrin complex adaptor protein synaptotagmin 1 (Syt 1) appears to be essential to this process, but little is known about its expression in intestinal epithelial cells or interaction with NHE3. The intestinal epithelial expression and apical location of Syt 1 were determined by Syt 1 mRNA profiling and immunolocalization. Tandem mass spectrometry was used for protein identification. BS(3)-crosslinking suggested that NHE3 and Syt 1 were in a membrane complex following cAMP stimulation of Caco2BBE cells. To investigate the regulation of NHE3 appearance in a Syt 1...

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  An imported case of P. falciparum malaria presenting as black water fever with acute renal failure

Summary: This is a report of a case of blackwater fever in a 28-year-old Nigerian man who was admitted to hospital with fever, jaundice and passing dark urine. Abdominal examination revealed splenomegaly and an examination of the peripheral smear of the patient showed the ring form of the trophozoites of Plasmodium falciparum (P. falciparum). Serum creatinine was 200μmol/L. Treatment with quinine and doxycycline was started and intravenous fluids were administered with close monitoring of the urine output and serum electrolytes. Due to the alarming amount of fluid accumulation and his exacerbated azotaemia the decision was made to haemodialyse the patient; the patient required five haemodialysis sessions during his stay in the hospital. He was discharged on the sixteenth day after admissi...

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  Removal of intracanal smear layer by doxycycline: SEM analysis

The aim of this study was to verify the efficacy of doxycycline used alone or in association with sodium hypochlorite on smear layer removal. The canals of extracted human teeth were instrumented using a stepback technique. At the end of preparation, they were irrigated with doxycycline (Group 1), ethylenediaminetetraacetic acid + sodium hypochlorite (Group 2), doxycycline + sodium hypochlorite (Group 3) and water (Group 4). The dentinal wall of the cervical, middle and apical thirds was graded according to the amount of remaining debris and smear layer. The results were analysed using the Kruskal[ndash]Wallis test (P < 0.05). In Groups 2 and 4, the dentine was completely free and covered with smear layer, respectively. The results from the cervical and middle thirds of Group 3 were worse ...

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  Efracea 40mg Modified Release Capsules (doxycycline) - New formulation

Source: MIMS Area: Other Library Updates > SPC Changes EFRACEA, a new formulation of doxycycline, is indicated to reduce papulopustular lesions in adult patients with facial rosacea. The daily dose is one 40 mg MR capsule in the morning. (Source: NeLM - Dermatology)

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  Functional characterization of THY1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma

In this study, a tetracycline-inducible expression vector, pETE-Bsd, was used to obtain stable transfectants of THY1. The stringent in vivo tumorigenicity assay results show that the activation of THY1 suppresses tumor formation of HONE1 cells in nude mice, and the tumor formation ability was restored in the presence of doxycycline (a tetracycline analog), when the gene is shut off. Functional inactivation of this gene is observed in all the tumors derived from the tumorigenic transfectant. The tumor suppressive effect could be repressed by knockdown of THY1 expression in nontumorigenic microcell hybrids. Further studies indicate that expression of THY1 inhibits HONE1 cell growth in vitro by arresting cells in G0/G1 phase. It greatly reduces the ability for anchorage-independent growth. Th...

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  Locally Delivered Doxycycline has no Long-Term Benefits for Periodontal Health

Article Title and Bibliographic Information: Locally delivered doxycycline during supportive periodontal therapy: a 3-year study.Bogren A, Teles RP, Torresyap G, Haffajee AD, Socransky SS, Wennström JL.J Periodontol 2008;79(5):827-35.Reviewer: Francis G. Serio, DMD, MS, MBAPurpose/Question: The authors studied the long-term clinical and microbiological effects (3 years) of yearly applications of locally delivered doxycycline as an adjunct to mechanical supportive periodontal therapy (SPT) compared with conventional SPT without locally delivered antibiotic.Source of Funding: Government - National Institute of Dental and Craniofacial Research (USA)Type of Study/Design: Multicenter, randomized controlled clinical trial with calibrated and blinded examiners.Level of Evidence: Level 1: Good-qu...

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  Determination of the mutant selection window for clindamycin, doxycycline, linezolid, moxifloxacin and trimethoprim/sulfamethoxazole against community-associated meticillin-resistant Staphylococcus aureus (MRSA)

In this study, mutant prevention concentration (MPC) testing was used to investigate the propensity for future resistance induction in CA-MRSA by clindamycin, doxycycline, linezolid, moxifloxacin and trimethoprim/sulfamethoxazole. Four CA-MRSA isolates [two each of clones USA300 (10841 and NRS384) and USA400 (2833 and NRS123)] were tested as well as a single hospital-acquired strain (NRS385). A variable hierarchy of the tested antimicrobials with respect to the percentage of the dosage interval that concentrations fall within the mutant selection window (%TMSW) was determined, with all antimicrobials achieving %TMSW values >20%. Against the hospital-acquired strain, all antimicrobials except linezolid (%TMSW=74.10%) and moxifloxacin (%TMSW=21.65%) are predicted to attain concentrations bel...

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  Doxycyline inhibits mononuclear cell-mediated connective tissue breakdown

In this study, we show that doxycycline, originally developed as a broad-spectrum tetracycline antibiotic (and, more recently, as a nonantimicrobial therapy for chronic inflammatory periodontal and skin diseases), can inhibit extracellular matrix degradation in cell culture mediated by human peripheral blood-derived monocytes/macrophages. The mechanisms include downregulation of cytokines and MMP-9 protein levels and the inhibition of the activities of both collagenase and MMP-9. These pleiotropic, but nonantibiotic, effects of doxycycline explain, at least in part, its therapeutic potential for various chronic inflammatory diseases including periodontitis, and may reduce the risks of systemic diseases (e.g. CVDs, less manageable diabetes) associated with this and other local diseases. (So...

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  Distinct Functions of Airway Epithelial NF-{kappa}B Activity Regulate Nitrogen Dioxide-induced Acute Lung Injury.

In this study, we investigated whether manipulation of NF-kappaB activity in lung epithelium affected severe lung injury induced by NO2 inhalation. Wild type C57BL/6J, CC10-IkappaBalphaSR transgenic mice with repressed airway epithelial NF-kappaB function, or transgenic mice expressing a doxycycline-inducible constitutively active I kappa B kinase beta (CC10-rTet-CAIKKbeta) with augmented NF-kappaB function in airway epithelium were exposed to toxic levels of 25 ppm or 50 ppm NO2 for six hours a day for one or three days. In wild type mice, NO2 caused NF-kappaB activation in airway epithelium after 6 hours, and after three days resulted in severe acute lung injury characterized by neutrophilia, peribronchiolar lesions, and increased protein, lactate dehydrogenase and inflammatory cytokines...

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  TAp63 induces senescence and suppresses tumorigenesis in vivo

Authors: Xuecui Guo, William M. Keyes, Cristian Papazoglu, Johannes Zuber, Wangzhi Li, Scott W. Lowe, Hannes Vogel & Alea A. Mills p63 is distinct from its homologue p53 in that its role as a tumour suppressor is controversial, an issue complicated by the existence of two classes of p63 isoforms. Here we show that TAp63 isoforms are robust mediators of senescence that inhibit tumorigenesis in vivo. Whereas gain of TAp63 induces senescence, loss of p63 enhances sarcoma development in mice lacking p53. Using a new TAp63-specific conditional mouse model, we demonstrate that TAp63 isoforms are essential for Ras-induced senescence, and that TAp63 deficiency increases proliferation and enhances Ras-mediated oncogenesis in the context of p53 deficiency in vivo. TAp63 induces senescence indepe...

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  Why hasn't this tender nodule responded to an antibiotic?

A 63-year-old woman presents with a tender nodule of 10 days’ duration on the dorsum of her right hand. She does not remember any specific injury, but she does do her own housework. Another physician prescribed doxycycline, 100 mg bid; however, the nodule has persisted. You order a bacterial culture. (Source: Consultant Live)

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  Expression of Activated STAT5 in Neoplastic Mast Cells in Systemic Mastocytosis. Subcellular Distribution and Role of the Transforming Oncoprotein KIT D816V.

We examined the expression of pSTAT5 in neoplastic mast cells in systemic mastocytosis and asked whether the disease-related oncoprotein KIT D816V is involved in STAT5 activation. As assessed by immunohistochemistry using the anti-pSTAT5 antibody AX1, neoplastic mast cells were found to display pSTAT5 in all SM patients examined (n = 40). Expression of pSTAT5 was also demonstrable in the KIT D816V-positive mast cell leukemia cell line HMC-1. Using various staining-protocols, pSTAT5 was found to be located in both the cytoplasmic and nuclear compartment of mast cells. To define the functional role of KIT D816V in STAT5-activation, Ba/F3 cells with doxycycline-inducible expression of KIT D816V were used. In these cells, induction of KIT D816V resulted in an increased expression of pSTAT5 wit...

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  In vitro susceptibility of Achromobacter spp. isolates: comparison of disk diffusion, Etest and agar dilution methods

In this study, we analysed the antimicrobial susceptibility of 92 strains of Achromobacter spp. isolated from clinical samples to 18 antimicrobial agents. The disk diffusion method and Etest were compared with the agar dilution method, and the breakpoints of susceptibility and resistance for the disk diffusion method for the antimicrobials tested were determined. The most active antibiotics were piperacillin, piperacillin/tazobactam and the carbapenems. By applying the linear least-squares regression method, breakpoints could be established for antibiotics active against this genus such as imipenem, meropenem, ertapenem and trimethoprim/sulfamethoxazole (SXT). Other active antibiotics, such as piperacillin and minocycline, could be tested by the Etest method. The less active antibiotics su...

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  DOXYCYCLINE HYCLATEcapsule, Gelatin Coated [Apace Packaging LLC]

Updated Date: Nov 4, 2009 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))

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  LAC and LOAD: 4-Drug Regimen Better at Eliminating H. Pylori Than Standard 3-Drug Treatment

LOAD (levofloxacin, omeprazole, nitazoxanide, and doxycycline) is more effective at eradicating Helicobacter pylori than LAC (lansoprazole, amoxicillin, and clarithromycin). Medscape Medical News (Source: Medscape Today Headlines)

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  LAC and LOAD: 4-Drug Regimen Better at Eliminating H Pylori Than Standard 3-Drug Treatment

LOAD (levofloxacin, omeprazole, nitazoxanide, and doxycycline) is more effective at eradicating Helicobacter pylori than LAC (lansoprazole, amoxicillin, and clarithromycin). Medscape Medical News (Source: Medscape Gastroenterology Headlines)

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  Doxycycline-Mediated Inhibition of Choroidal Neovascularization [Biochemistry and Molecular Biology]

Doxycycline effectively hampers the progression of experimental CNV, increases PEDF levels, and inhibits MMP-2, MMP-9, and gelatinolytic activities in situ. The results suggest that orally administrated doxycycline can reach the choroid to attenuate proteolytic enzymes that remodel Bruch's membrane and promote antiangiogenic PEDF to prevent neovascularization. (Source: Investigative Ophthalmology)

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  Loading and release of doxycycline hyclate from strontium-substituted calcium phosphate cement

Abstract: Novel Sr-substituted calcium phosphate cement (CPC) loaded with doxycycline hyclate (DOXY-h) was employed to elucidate the effect of strontium substitution on antibiotic delivery. The cement was prepared using as reactants Sr-substituted β-tricalcium phosphate (Sr-β-TCP) and acidic monocalcium phosphate monohydrate. Two different methods were used to load DOXY-h: (i) the adsorption on CPC by incubating the set cement in drug-containing solutions; and (ii) the use of antibiotic solution as the cement liquid phase. The results revealed that the Sr-substituted cement efficiently adsorbs the antibiotic, which is attributed to an enhanced accessibility to the drug-binding sites within this CPC. DOXY-h desorption is influenced by the initial adsorbed amount and the cement matrix type...

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  Sun Defense Kit for Acne-Prone Skin

Acne patients may be at increased risk for sun sensitivity and skin damage, depending on their treatment regimen. The Avidoxy DK defence kit offers products that provide sun protection for acne suffers without exacerbating their condition. The kit consists of 100-mg doxycycline tablets (available by prescription only) to help destroy acne-causing bacteria, plus two free products: defence acne wash and defence solare SPF 30. The acne wash contains 2% salicylic acid to promote cleansing and exfoliation, and it is oil-free and alcohol-free. The defence solare SPF is an oil-free, noncomedogenic, moisturizing sunblock that contains antioxidants and zinc oxide to provide broad-spectrum sun protection for acne-prone skin. (Source: Skin and Allergy News)

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  Multicenter GeoSentinel analysis of rickettsial diseases in international travelers, 1996-2008.

Authors: Jensenius M, Davis X, von Sonnenburg F, Schwartz E, Keystone JS, Leder K, Lopéz-Véléz R, Caumes E, Cramer JP, Chen L, Parola P, We investigated epidemiologic and clinical aspects of rickettsial diseases in 280 international travelers reported to the GeoSentinel surveillance Network during 1996-2008. Of these 280 travelers, 231 (82.5%) had spotted fever (SFG) rickettsiosis, 16 (5.7%) scrub typhus, 11 (3.9%) Q fever, 10 (3.6%) typhus group (TG) rickettsiosis, 7 (2.5%) bartonellosis, 4 (1.4%) indeterminable SFG/TG rickettsiosis, and 1 (0.4%) human granulocytic anaplasmosis. One hundred ninety-seven (87.6%) SFG rickettsiosis cases were acquired in sub-Saharan Africa and were associated with higher age, male gender, travel to southern Africa, late summer season trav...

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  [Successful use of combined corticosteroids and rituximab in the treatment of recalcitrant epidermolysis bullosa acquisita.]

We report a new case of successful treatment of EBA using rituximab (anti-CD20 antibody) without relapse after 1 year of follow-up. CASE REPORT: A 76-year-old man was seen for blisters of the skin and mucosa, atrophic scars and milia on areas of friction. The diagnosis of EBA was made on the basis of histological and immunohistochemical criteria. The patient was unsuccessfully treated with topical steroids, dapsone, topical tacrolimus, systemic steroids, mycophenolate mofetil, doxycycline and methotrexate. Four weekly infusions of rituximab of 375mg/m(2) body area were performed, combined with systemic steroids: they proved beneficial within 3 weeks, with a noticeable improvement and no further blisters at 7 months. After 1 year of follow-up, the skin disease is still stable with 5mg/day o...

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  Sex affects the steady-state pharmacokinetics of primaquine but not doxycycline in healthy subjects.

Authors: Binh VQ, Chinh NT, Thanh NX, Cuong BT, Quang NN, Dai B, Travers T, Edstein MD We evaluated whether sex affects the steady-state pharmacokinetics of the antimalarial drugs, primaquine and doxycycline, in healthy subjects. Seventeen male and 17 female healthy Vietnamese subjects were administered 30 mg (base) of primaquine daily for 14 days. After a 2-week washout period, 14 male and 14 female subjects were administered 100 mg (base) of doxycycline daily for 14 days. Women had significantly higher median values of C(max) (212 versus 122 ng/mL, P< 0.001) and AUC(0-24) (1,909 versus 917 ng . h/mL, P < 0.001) of primaquine compared with men. Other than a longer t(max) in women, no sex-related differences were seen in the pharmacokinetics of doxycycline. The primaquine pharmac...

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  Cleavage of functional IL-2 receptor alpha chain (CD25) from murine corneal and conjunctival epithelia by MMP-9

Conclusions: Our results indicate that functional IL-2R is produced by the ocular surface epithelia and that CD25 is proteolytic cleaved to its soluble form by MMP-9, which increases in desiccating stress. These findings provide new insight into IL-2 signaling in mucosal epithelia. (Source: Journal of Inflammation)

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  Bordetella bronchiseptica pneumonia in a kidney–pancreas transplant patient after exposure to recently vaccinated dogs

This report expands the spectrum of immunocompromised hosts to include renal[ndash]pancreas transplant patients who have developed infection from B. bronchiseptica, while illustrating the risks associated with animal contacts and attenuated vaccines in the immunosuppressed population. (Source: Transplant Infectious Disease)

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  Antibiotics in Addition to Systemic Corticosteroids for Acute Exacerbations of COPD.

CONCLUSIONS: While equivalent to placebo on clinical success at day 30, doxycycline showed superiority on clinical success and clinical cure at day 10, microbiological success, the use of open label antibiotics and symptoms. (ClinicalTrials.gov #NCT00170222.). PMID: 19875685 [PubMed - as supplied by publisher] (Source: Am J Respir Crit Car...)

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  Evidence for the involvement of matrix metalloproteinases in the cardiovascular effects produced by nicotine.

We examined whether nicotine induces the release of MMP-2 and MMP-9 by rat smooth muscle cells (SMC), and whether doxycycline (non-selective MMP inhibitor) inhibits the vascular effects produced by nicotine. SMC were incubated with nicotine 0, 50, and 150 nM for 48h. MMP-2 and MMP-9 levels in the cells supernatants were determined by gelatin zymography. The acute changes in mean arterial pressure caused by nicotine 2mumol/kg (or saline) were assessed in rats pretreated with doxycycline (or saline). We also examined whether doxcycline (30mg/Kg, i.p., daily) modifies the effects of nicotine (10mg/kg/day; 4weeks) on the endothelium-dependent relaxations of rat aortic rings. Aortic MMP-2 levels were assessed by gelatin zymography. Aortic gelatinolytic activity was assessed using a gelatinolyti...

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  Combination of alpha-1 antitrypsin and doxycycline suppresses collagen-induced arthritis

Rheumatoid arthritis (RA) is a complex disease characterized by autoimmune inflammation and joint destruction. Despite recent advances in RA treatment, current therapies require further improvement to overcome adverse events and ineffectiveness in some cases. By targeting different pathways/molecules using drug combinations, a better treatment can be obtained, whereas adverse events are reduced. In order to develop a new treatment option, the present study employs a gene therapy-based combination therapy using doxycycline and human alpha-1 antitrypsin (hAAT).DBA/1 mice were immunized with type II collagen to induce arthritis. Four weeks before immunization, they received a doxycycline containing diet and a single injection of adeno-associated virus vector expressing hAAT under the control ...

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  New Combination Therapy Looks Promising Against Ulcer Bacteria

Helicobater pylori, a bacteria implicated in peptic ulcers and gastritis, was eradicated in 95 percent patients who took a 7-day course of combination therapy with levofloxacin, omeprazole, nitazoxanide (Alinia®) and doxycycline (LOAD) compared to eradication in only 80.9 percent of patients on lansoprazole, amoxicillin and clarithromycin (LAC) for seven days. (Source: Health News from Medical News Today)

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  Pretreatment of Epithelial Cells with Rifaximin Alters Bacterial Attachment and Internalization Profiles.

Authors: Brown EL, Xue Q, Jiang ZD, Xu Y, Dupont HL Rifaximin is a poorly absorbed semi-synthetic antibiotic derivative of rifampin licensed for use in the treatment of traveler's diarrhea. Rifaximin reduces symptoms of enteric infection, often without pathogen eradication and with limited effects on intestinal flora. Epithelia cells (HEp-2, larynx; HCT-8, ileocecal; A549, lung and HeLa, cervical) were pretreated with rifaximin (or control antibiotics) prior to the addition of enteroaggregative Echerichia coli (EAEC). EAEC adherence was significantly reduced following rifaximin pretreatment compared to pretreatment with rifampin or doxycycline for 3 of 4 cell lines tested. The rifaximin-mediated changes to epithelial cells were explored further by testing attachment and internalization...

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  Relapse-associated autoantibodies to BP180 in a patient with anti-p200 pemphigoid

We report a 70-year-old man with haemorrhagic blisters, widespread crusted erosions, and the immunopathological characteristics of anti-p200 pemphigoid. Treatment with doxycycline, topical corticosteroids and immunoadsorption led to rapid clinical remission. However, 19 weeks later, a relapse occurred with generalized itchy urticarial erythema and tense blisters. At this time, both strong dermal and epidermal IgG staining was detected by indirect immunofluorescence microscopy on salt-split skin, and autoantibodies against both p200 and the 16th noncollagenous (NC16A) domain of BP180 were found. Interestingly, the relapse was associated not only with the detection of autoantibodies to a second autoantigen (BP180), but also with an altered clinical phenotype. This case was a unique occasion ...

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  Use of short message service (SMS) to improve malaria chemoprophylaxis compliance after returning from a malaria endemic area

Conclusions: SMS did not increase malaria chemoprophylaxis compliance above baseline, likely because the persons did not benefit from holidays after the return and stayed together. So the reminder by SMS was noted by all subjects enrolled to the study. Another study should be done to confirm these results on soldiers going on holidays from employment after return or with individual travellers. (Source: Malaria Journal)

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  Doxycycline loaded poly(ethylene glycol) hydrogels for healing vesicant-induced ocular wounds.

In conclusion, the current studies demonstrate that the doxycycline-PEG hydrogels accelerate corneal wound healing after vesicant injury offering a therapeutic option for ocular mustard injuries. PMID: 19853296 [PubMed - as supplied by publisher] (Source: Biomaterials)

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  [Clinical Picture] Bone marrow involvement in Q fever—detection by fluorine-18-labelled fluorodeoxyglucose PET

A 60-year-old man was admitted to hospital with a 2-week history of fever, rigors, and headache. Apart from hypertension, his past medical history was unremarkable. He travelled abroad frequently, and had visited China where he had been exposed to a herd of goats 2 months before the current illness. On examination, he was pyrexial (38·5°C) and his liver was enlarged. His full blood count was normal, but his C-reactive protein was raised. Serum transaminases and alkaline phosphatase were mildly raised. Preliminary screening for auto-antibodies was negative. Initial parasitic and virological screening was also negative. Cerebrospinal fluid showed a mild increase in protein and a reduced glucose. MRI of his head was normal as was CT of his chest, abdomen, and pelvis. Indium-111-labelled leu...

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  In vitro activity of mirincamycin (U24729A) in Plasmodium falciparum isolates from Gabon.

Authors: Held J, Westerman R, Kremsner PG, Mordmüller B We assessed the in vitro activity of mirincamycin, a lincosamide antibiotic, against Plasmodium falciparum clinical isolates from Gabon. Growth was determined by HRP2-ELISA using an adapted protocol with prolonged incubation time (6 days) to account for antibiotic induced delayed death. Mirincamycin's cis and trans isomers are more active (median IC50: 3.2 nM and 2.6 nM) than the comparator drugs clindamycin (IC50: 12 nM) and doxycycline (IC50: 720 nM) and therefore further clinical development is promising. PMID: 19841147 [PubMed - as supplied by publisher] (Source: Antimicrobial Agents and Chemotherapy)

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  Inhibition of matrix metalloproteinase activity in vivo protects against vascular hyporeactivity in endotoxemia.

This study demonstrates the in vivo protective effect of the MMP inhibitor doxycycline against the development of vascular hyporeactivity in endotoxemic rats. Key words: endotoxemia, vascular hyporeactivity, matrix metalloproteinase, doxycycline. PMID: 19837953 [PubMed - as supplied by publisher] (Source: American Journal of Physiology. Heart and Circulatory Physiology)

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  Aryl hydrocarbon nuclear translocator (hypoxia inducible factor 1[beta]) activity is required more during early than late tumor growth

c4 is a derivative of the mouse hepatoma cell line, Hepa-1, that harbors a mutation in the aryl hydrocarbon receptor nuclear translocator gene (Arnt, or hypoxia inducible factor 1[beta] [HIF-1[beta]]) leading to loss of activity. Clone 3 cells were generated by introducing a doxycycline-repressible Arnt expression vector into c4 cells. Clone 3 cells were injected subcutaneously into immunosuppressed mice, which were treated with doxycyline (a) throughout the growth of the subsequent tumor xenografts, or (b) from day 7 through to the end of the experiment (day 30), or not treated (c). Tumors in all groups grew exponentially between days 14 and 30, and at rates that were indistinguishable from each other. However, tumors in group a were smaller than those of the other two groups throughout t...

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  Flucloxacillin, still the empirical choice for putative Staphylococcus aureus infections in intensive care units in the Netherlands

Conclusions The empirical choice of flucloxacillin in the case of putative S. aureus infections in patients admitted to ICUs in the Netherlands is still justified. (Source: Journal of Antimicrobial Chemotherapy)

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  Doxycycline: Severe thrombocytopenia: case report

(Source: Reactions)

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  Drugs for Malaria Prevention in Travelers Compared

Atovaquone-proguanil and doxycycline appear to be relatively well-tolerated for malaria prophylaxis in travelers, and are less likely to cause neuropsychiatric effects than mefloquine, according to research published online Oct. 7 in the Cochrane Database of Systematic Reviews. (Source: Modern Medicine)

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  Two Malaria Drugs for Travelers Have Fewer Side Effects

10/06/2009, Cochrane Library, A new research review finds that a combination drug and the common antibiotic doxycycline allow travelers to fend off malaria with the fewest side effects in areas where the parasite is resistant to a widely accepted preventive treatment. (Source: Health Behavior News Service)

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  A randomized double-blind comparison of moxifloxacin and doxycycline/metronidazole/ciprofloxacin in the treatment of acute, uncomplicated pelvic inflammatory disease

This multicentre, double-blind study was undertaken to demonstrate non-inferiority of once-daily oral moxifloxacin compared with combination therapy in the management of acute, uncomplicated pelvic inflammatory disease (PID). Women aged ≥18 years with PID were randomized to receive moxifloxacin (400 mg once daily) for 14 days or comparator treatment (doxycycline [100 mg twice daily] plus metronidazole [400 mg three times daily] for 14 days, plus one single 500-mg ciprofloxacin dose). Of the 434 valid per protocol (PP) patients, the overall clinical success rates at 2–14 days post-therapy were 96.6% (moxifloxacin) and 98.0% (comparator); moxifloxacin was non-inferior to the comparator regimen both in the PP (95% confidence interval [CI]: –4.5, 1.6) and intent-to-treat (95% CI...

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  Doxycycline Shows Efficacy Against M. Perstans

Doxycycline is effective against infection with the filarial parasite Mansonella perstans, according to results of study conducted in Mali and reported October 8th in The New England Journal of Medicine. Reuters Health Information (Source: Medscape Infectious Diseases Headlines)

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  Two Malaria Drugs For Travelers Have Fewer Side Effects

A new review of existing research finds that a combination drug and the common antibiotic doxycycline allow travelers to fend off malaria with the fewest side effects in areas where the parasite is resistant to a widely accepted preventive treatment. (Source: Health News from Medical News Today)

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Top Viagra Store

A first shop in the series of one-pill shops is rightfully viagra-targeted. The index page offers only Viagra, both generic and original.It is possible to purchase other ED products from other pagers, but the main accent is on Viagra.

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Indian Meds

A most recent indian-themed, ethnic site. Includes generic and original (brand) products. al our standard navigational tools and support options are available. This site converts nicely on general pharmaceutical traffic as it has products for vurtually any need.

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Friendly ED Pharmacy

Many ED customers are anxious about the status their orders. This special friendly site is here to solve the problem. "State of the art" design features high usability and clearly visible support options. All this helps to satisfy even the most demanding customer and results in increased sales ratio.

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Popular ED Products

This ED site offers simplistic design that gets right to the point - this IS the site for ED. Popular ED list the top selling products and converts best on ED-targeted, pill-specific traffic.

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Perfect ED Store

This site is just right for ED customers. All ED products are conveniently listed at main page, allowing to make an easy choice. Our regular Sample Pills Offer is emphasized here. Customers can try new products and are likely to return for more refills.

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Worlds Best Pharmacy

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Delhi Drugs

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Indian Generics

Many customers are looking for Indian drugs, and this site is designed specifically for such customers. It includes many generic products, modern design, translated into 7 languages and prices in both USD and Euros. Also it uses our latest shopping engine and billing page.

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